白细胞介素-12增强HBsAg疫苗免疫应答的实验研究

HBsAg Immune Response on Mouse Models Enhanced by Recombinant Murine Interluekin-12

目的研究白细胞介素-12（IL-12）对乙肝病毒表面抗原（HBsAg）疫苗的免疫增强作用。方法选用C57BL／6J小鼠，使用不同剂量的重组鼠白细胞介素-12（rmlL-12）和HBsAg联合给药，检测给药前后血清中IFN-1，的浓度以及给药后小鼠脾脏淋巴细胞对HBsAg刺激的免疫应答能力的变化。结果高剂量HBsAg和高剂量rmIL-12联合两次给药后，血清中IFN-γ浓度明显高于单独给HBsAg组或rmIL-12组（P〈0．05）；高剂量联合给药组的小鼠脾脏淋巴细胞经HBsAg体外刺激后诱生IFN-γ的能力明显高于单独给HBsAg组或rmlL-12组（P〈0．05），流式细胞检测分析表明IFN-γ主要来源于CD4^＋和CD8^＋的T细胞。结论rmlL-12能快速明显地提高C57BL／6J小鼠对HBsAg的特异性免疫应答能力，为IL-12和HBsAg在临床上联合治疗慢性乙肝感染提供了理论依据。

Objective To investigate the enhanced immunity of hepatitis B surface antigen （HBsAg） vaccine in response to interleukin-12 （IL-12） stimulation. Method C57BL/6J mice were injected with rmlL-12 plus HBsAg combination, after which the serum IFN-γ level as well as the response of murine spleen lymphocytes to HBsAg was detected. Results Serum IFN-γ level in high dose combination of HBsAg and rmlL-12 group after two injections was significant higher than that in HBsAg or rmlL-12 alone （P〈0.05）. The ability of murine spleen lymphocytes producing IFN-γ in response to HBsAg was significantly improved （P〈0.05）, and IFN-y mainly originated from CD4^＋ ＆ CD8^＋ T cells detected by flow cytometric analysis. Conclusion The immunity response of C57BL/6J mice to HBsAg can be rapidly and specifically enhanced by rmlL-12, which may be a reference of IL-12 plus HBsAg clinical application on CHB infection.