实时定量PCR检测GVHD患者TCR V_γ亚家族基因表达水平

Quantitative real-time PCR for detecting the expression levels of TCR V_γ subfamilies in patients with graft-versus-host disease following allogenic hematopoietic stem cell transplantation

目的了解异基因造血干细胞移植后移植物抗宿主病(GVHD)疾病状态下T细胞受体(TCR) Vγ亚家族T细胞水平的变化情况。方法采用实时荧光定量聚合酶链反应对18例GVHD患者外周血单个核细胞TCR VγI～III亚家族基因表达水平进行相对定量检测;12例健康成人外周血样本作为对照。结果GVHD患者外周血中TCR VγII的表达量显著低于正常对照;GVHD患者TCR VγI～III亚家族的表达模式也发生了改变,TCR VγII的表达量比TCR VγI或TCR VγIII的表达量低。结论TCR VγII亚家族的表达低下可能与GVHD的发病相关。

Objective To investigate the expression ofT cell receptor （TCR） VT genes in patients with graft-versus-host disease （GVHD） after allogenic hematopoietic stem cell transplantation （allo-HSCT）. Methods The expression levels of the TCR Vγ Ⅰ-Ⅲ genes in peripheral blood mononuelear cells （PBMNCs） from 18 patients with GVHD following allo-HSCT were determined using real-time fluorescence quantitative PCR, with 12 healthy individuals serving as the normal controls. Results The expression level of TCR VγⅡ gene in the PBMNCs from patients with GVHD was significant lower than that in the normal controls. The expression patterns of TCR VγⅠ-Ⅲ subfamilies also underwent alterations in patients with GVHD, and the expression level of TCR VγⅡ gene was significantly lower than that of TCR VγⅠ gene or TCR VγⅢ gene. Conclusion The low expression ofTCR VγⅡ subfamily might be related to the pathogenesis of GVHD in patients receiving allo-HSCT.