钩吻素子对小鼠H22实体瘤抑制作用的实验研究

Antineoplastic effect of koumine in mice bearing H22 solid tumor

目的观察钩吻素子(koumine,Kou)对荷H22肝癌实体瘤小鼠的作用及实验动物免疫功能的影响。方法观察移植性H22肝癌BALB/c裸鼠实体瘤模型给药后肿瘤的生长情况,小鼠脾脏重量及血液细胞数目变化。结果Kou能抑制H22实体瘤的生长,随剂量升高而瘤重降低;Kou组动物的脾重指数及血细胞数目与对照组相比无显著性差异,但高于5-Fu组,提示Kou可能对实验动物的免疫功能没有抑制作用。结论Kou能显著抑制荷H22肝癌实体瘤的BALB/c裸鼠后的肿瘤生长,且对免疫系统无明显抑制作用。

Objective To investigate the antitumor effects ofkoumine in mice bearing H22 solid tumor and its effect on the immune system of the mice. Methods The changes in spleen and tumor weights and blood cell count were observed after koumine treatment in BALB/c athymic mice bearing H22 solid tumor, using normal saline solution and 5-Fu as the controls. Results Koumine significantly inhibited the tumor growth in a dose-dependent manner. The spleen index and blood cell counts in koumine group showed no significant differences from those in the saline control group, but higher than those in 5-Fu group. Conclusion Koumine can significantly inhibit the growth of H22 solid tumor without obvious inhibitory effect on the immune system in mice.