非小细胞肺癌中Aurora-B的表达及其临床意义

Expression of Aurora-B in non-small cell lung cancer and its clinical significance

目的探讨丝氨酸/苏氨酸激酶家族成员Aurora-B在非小细胞肺癌(NSCLC)中的表达及其意义。方法应用免疫组织化学方法检测NSCLC组织中Aurora-B蛋白的表达,其中肿瘤组织121例,癌旁组织41例,Ⅰ期86例,Ⅱ～Ⅲ期35例;应用逆转录聚合酶链式反应法(RT-PCR)检测NSCLC细胞株A549、H460及H1299等细胞株中Aurora-BmRNA的表达;应用Western印迹法检测上述细胞株中Aurora-B蛋白的表达。结果Aurora-B蛋白在121例NSCLC组织中的表达率为77.7%(94/121),癌旁组织中的表达率为9.8%(4/41),差异有统计学意义(P<0.01);Aurora-B蛋白的表达在不同病理类型之间(腺癌、鳞癌及细支气管肺泡癌)的差异也有统计学意义(P<0.05),在鳞癌中表达明显上调;与NSCLC的淋巴结是否转移以及肿瘤的分化程度也密切相关(P<0.05);而与患者的性别、年龄等因素则无明显相关性(P>0.05);在NSCLC细胞株A549、H460及H1299中均存在Aurora-BmRNA及蛋白的表达,其中以A549细胞株的表达水平为最高。结论在NSCLC组织及细胞株中存在Aurora-B的表达;Aurora-B蛋白表达在NSCLC的分化、转移和发展中具有重要作用;在多种NSCLC细胞株中Aurora-B的表达水平存在一定差别。

Objective To study the expression of Aurora-B in non-small cell lung cancer （NSCLC） tissues and NSCLC cell lines. Method Aurora-B expression was examined using immunohistochemical SP method in 91 stage Ⅰ and 69 stage Ⅱ-Ⅲ NSCLC tissues and 40 adjacent tissues. The mRNA and protein expressions of Aurora-B in NSCLC cell lines （A549, H460 and H1299） were examined by RT-PCR and Western blotting, respectively. Results The protein expression of Aurora-B was detected in 77.7% （94/121） of the tumor tissues and 9.8% （4/41） of the adjacent tissues, showing a significant difference between them （P〈0.01）. The positivity rate of Aurora-B protein was not related with the gender and age of NSCLC patients, but with lymph node metastasis, differentiation and histological type ofNSCLC （P〈0.05）. Aurora-B was expressed in all the NSCLC cell lines （A549, H460 and H1299） at both mRNA and protein levels. A549 cells showed the highest expression of Aurora-B. Conclusion Aurora-B protein is highly expressed in NSCLC tissues and cell lines, and may play a crucial role in the invasion, metastasis and development of NSCLC. The mRNA and protein expression levels of Aurora-B differ significantly between different NSCLC cell lines.