汉族人血小板GPⅡb HPA-3基因多态性与冠心病的相关性研究

Association between platelet membrane glycoprotein IIb polymorphism and coronary heart disease in Han people

目的:观察血小板膜糖蛋白(GP)Ⅱb人类血小板抗原-3(HPA-3)基因多态性,在北京河北地区汉族人中各基因型的分布频率,分析该多态性与冠心病易感性的相关性。方法:本研究采用病例对照设计,筛选符合冠心病、健康人入选标准的212例冠心病患者及106例健康对照人群为研究对象,记录冠脉造影病变支数,采用TaqMan探针技术检测HPA-3基因多态性。结果:大于45岁的人群中,至少含有1个HPA-3b等位基因者较HPA-3a/3a原生型纯合子,冠心病人(72.4%/27.6%)多于健康人(57.1%/42.9%),不同基因型在冠心病组和健康对照组两组间的分布具有显著差异(P<0.05);冠心病患者不同病变支数基因型构成无显著差异(P>0.05)。以是否患冠心病为因变量的二分类Binary Logistic回归分析,校正年龄、性别、体重指数对冠心病的影响后,结果显示HPA-3多态位点基因型与冠心病发病密切相关,含有至少1个HPA-3b等位基因者较HPA-3a/HPA-3a纯合型发生冠心病的危险性是2.105倍。结论:GPⅡb的HPA-3多态位点是汉族人冠心病发病的独立危险因素,尤其在年龄>45岁的人群中表现明显。

AIM： To investigate the genotype distributions of HPA -3 in Hart people from Beijing and Hebei province, and to study the association of the platelet glycoprotein Ⅱb polymorphism with coronary heart disease （CHD）. METHODS： Two hundred and twelve patients with coronary heart disease and 106 healthy controls were enrolled in this case - control study. The number of occlusive coronary artery was performed in all subjects. The genotypes of HPA - 3 were determined by TaqMan probe technology. RESULTS ： In CHD patients （ older than 45 years） carriers of HPA - 3b were over - represented compared with healthy controls （P 〈 0. 05 ）. The prevalence of HPA - 3 genetic variants had no difference in CHD patients of different numbers of occlusive coronary artery. Results were statistically evaluated with Binary Logistic regression, which showed the HPA- 3 polymorphism was associated with an increased risk for CHD（ OR： 2. 105）. CONCLUSION ： The HPA - 3 polymorphism of GP Ⅱb is the risk factor of CHD in Han people, especially in patients older than 45 years.