水通道蛋白3在人前列腺组织中的表达及意义

Expression of aquaporin 3 in human prostate

目的:揭示水通道蛋白3(AQP3)在人前列腺移行区和外周区的表达及意义,为深入探讨AQP在人前列腺及疾病状态下的表达及生理作用奠定基础。方法:取前列腺组织标本5例,采用RT-PCR方法检测AQP3mRNA在前列腺组织的表达。同时应用Western blotting和免疫染色技术研究AQP3蛋白在前列腺组织的定位表达。结果:RT-PCR结果显示在前列腺的移行区和外周区均存在AQP3 mRNA表达。Western blotting结果显示人前列腺的移行区和外周区均特异表达AQP3蛋白,且移行区表达强于外周区。免疫组织化学和免疫荧光染色结果表明AQP3表达在前列腺移行区分泌细胞的细胞膜。结论:前列腺移行区分泌细胞表达AQP3提示,AQP3在前列腺液分泌过程中可能发挥重要的生理作用。

AIM： To determine the expression of aquaporin 3 （AQP3） in transitional and peripheral zone of human prostate tissue. METHODS ： AQP3 mRNA expression was analyzed in five prostate tissues by RT - PCR. AQP3 protein expression and localization were characterized by Western blotting and immuno - staining with polyclonal anti - AQP3 antibody. RESULTS ： The results of RT - PCR and Western blotting showed that AQP3 was expressed in transitional and peripheral zone of prostate tissue. The AQP3 protein expression in transitional zone was higher than that in peripheral zone. The results of immuno - staining indicated that AQP3 protein was expressed in secretory cells of prostate. CONCLUSION ： The AQP3 was expressed in prostate tissue, suggesting that AQP3 play an important role in the secretion of prostate liquid.