亚慢性砷暴露对雄性小鼠脑组织性基因Ddx3y和Uty表达的影响

Influence of subchronic exposure to arsenic on expressions of Ddx3y and Uty in brain of male mice

[目的]观察亚慢性砷暴露对雄性小鼠脑组织性基因Ddx3y和Uty表达的影响。[方法]SPF级雄性小鼠30只,按体重将小鼠随机分为对照组、低剂量染砷组(1 mg/LAs2O3)和高剂量染砷组(4 mg/LAs2O3),每组10只。自然饮水使小鼠染砷,连续染毒60 d后取脑。RT-PCR法检测雄性小鼠脑组织性基因Ddx3y和Uty的mRNA表达变化。[结果]与对照组比较,染砷组小鼠大脑髓质和小脑组织Ddx3y和Uty mRNA表达量明显增多,差异有显著性意义(P<0.05)。而大脑皮质性基因Ddx3y和Uty mRNA表达量明显减少,差异有显著性意义(P<0.05),尤其高剂量染砷组mRNA表达变化更明显。[结论]亚慢性砷暴露可下调雄性小鼠脑皮质性基因Ddx3y和Uty的mR-NA表达,同时上调髓质和小脑性基因Ddx3y和Uty的mRNA表达。

[Objective] To examine the influence of subchronic exposure to arsenic（As） on expressions of Ddx3y and Uty in the brain of mice.[Methods] Thirty male mice were divided into 3 groups of 10 each.The mice in group 1 received drinking water alone（control）,and group 2 or 3 received 1 or 4 mg/L arsenic trioxide.On the 60th day after As exposure,mice were decapitated and their brains were removed.Expressions of Ddx3y and Uty were examined in the different area ofbrain of mice exposed to As by RT-PCR.[Results] Compared to control group,the mRNA expressions of Ddx3y and Uty in cerebral medulla and cerebellum of mice increased significantly in the experimental groups（P〈0.05）.On the contrary,those in cerebral cortex decreased significantly in the experimental groups,especially in 4 mg/L arsenic trioxide group（P〈0.05）.[Conclusion] These results indicated that subchronic exposure to As down-regulates expressions of Ddx3y and Uty in the cerebral cortex and up-regulates those in cerebral medull a and cerebellum of mice.