对肝硬化腹水进行净化并超滤浓缩的实验研究

Study on endotoxin and bilirubin removal by CA/PEI affinity membrane and protein ultrafiltration by polysulfone hollow fiber membrane in ascites of cirrhosis

[目的]净化和优化肝硬化腹水使其更有利于浓缩回输治疗。[方法]采用CA/PEI亲和膜去除腹水胆红素、内毒素,并采用聚砜中空纤维膜超滤浓缩腹水,用内毒素测定仪、生化自动分析仪和免疫分析仪测定过膜前后各项指标。[结果]CA/PEI亲和膜吸咐前后腹水中的胆红素、内毒素均明显下降（P〈0.01）;胆红素吸附前（51.77±11.76）μmol/L;吸附后（21.18±6.83）μmol/L;内毒素吸附前（3.777±0.233）EU/mL,吸附后（0.341±0.322）EU/mL。白蛋白、离子、免疫球蛋白均无明显变化（P〉0.05）。腹水超滤浓缩5.5～6.5倍,蛋白质浓缩4.7～6.5倍,免疫球蛋白浓缩3倍,但离子无变化。[结论]用CA/PEI亲和膜可去除胆红素、内毒素,用聚砜中空纤维膜超滤浓缩能使腹水得到净化和蛋白浓缩。

[Objective] Purification and optimization of cirrhotic ascites is more helpful to reinfusion and treatment of ascites.[Methods] Endotoxin and bilirubin removal by CA/PEI affinity membrane and protein ultrafiltration by polysulfone hollow fiber membrane in ascites of cirrhosis were studied.The indexes of pre and post trans-membrane by endotoxin detector,biochemistry analyzer and immunity analyzer were measured.[Results] The endotoxin and bilirubin concentration in ascites were reduced with adsorption by CA/PEI affinity membrane（P〈0.01）.There were unchanged in albumin,ion and immunoglobulin（P〉0.05）.Before adsorptioned bilirubin is（51.77±11.76）μmol/L,after which is（21.18±6.83）μmol/L.Before adsorptioned endotoxin is（3.777±0.233）EU/mL,after which is（0.341±0.322）EU/mL.Ascites were concentrated 5.5 to 6.5 times by ultrafiltration,while the protein in ascites was concentrated 4.7 to 6.5 times and immunoglobulin was concentrated more than 3 times and ions were no change.[Conclusion] Ascites of purification and protein concentration may be obtained by the way of removal endotoxin and bilirubin with CA/PEI affinity membrane and ultrafiltration and concentration with polysulfone hollow fiber membrane.