三维实验法检测耐亚胺培南的铜绿假单胞菌产β-内酰胺酶

Three-dimensional method applied to detect beta-lactamases in imipenem-resistant pseudomonas aeruginosa

[目的]了解耐亚胺培南的铜绿假单胞菌产β-内酰胺酶的分布及分离率。[方法]采用K-B法进行药物敏感试验,筛选耐亚胺培南的铜绿假单胞菌;再进行改良三维实验,对菌株所产β-内酰胺酶进行分析。[结果]临床分离耐亚胺培南的铜绿假单胞菌40株中,单产超广谱β-内酰胺酶(ESBLs)9株(22.5%),单产高产染色体头孢菌素酶(AmpC)2株(5.0%),5株为超超广谱β-内酰胺酶(SSBL)(12.5%),3株产酶量过高或其他原因(7.5%),21株无酶活性(52.5%)。[结论]产β-内酰胺酶是耐亚胺培南铜绿假单胞菌的主要耐药机制之一。

[Objective] To analyze beta-lactamase in imipenem-resistant pseudomonas aeruginosa.[Methods] At first,the strains of imipenem-resistant P.Aeruginosa were selected with K-B susceptibility method.Then the three-dimensional method was taken to differentiate and analyze the various beta-lactamases.[Results] The enzymes produced by 40 strains imipenem-resistant P.Aeruginosa included extended spectrum beta-lactamases（ESBLs）（22.5%）.High level AmpC beta-lactamases（5.0%）,SSBL（12.5%）,enzyme production were too high or other reasons（7.5%） and no activity（52.5%）.[Conclusion] Production of beta-lactamases is one of the important reasons of imipenem-resistant P.aeruginosa.