摘要
目的：从蜱类媒介中分离斑点热群立克次体。方法：用鸡胚卵黄囊培养法直接分离。结果：自福建省宁化县林区麂体表捉获的越原血蜱（Ｈａｅｍｅｐｈｙｓａｌｉｓｙｅｎｉ）中分离到１株斑点热群立克次体，经微量补结试验和ＰＣＲ检测初步确定为西伯利亚立克次体（Ｒｉｃｋｅｔｓｉａｓｉｂｉｒｉｃａ）。
Objectives: To isolate spotted fever group rickettsiae from tick vectors. Methods: Directly isolated rickettsiae using embryonated hen's eggs. Results: A strain of spotted fever group rickettsiae was isolated from Haemaphysalis yeni collected from Muntiacus reevesi Ogil by captured in a forest area of Ninghua county, Fujian province. The isolate was primarily identified as Rickettsia sibirica by micro-complement-combination assay, polymerase chain reaction and other molecular biological techniques. Conclusion: These data provide more aetiological evidence for existence of North Asia Fever in Fujian province.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
1998年第4期287-290,共4页
Chinese Journal of Vector Biology and Control
基金
福建省科技项目资金
关键词
立克次体
分离
微量补结
PCR
越原血蜱
Spotted fever group rickettiae Complement combination assay Polymerase chain reaction(PCR) Haemaphysalis yeni
