摘要
为研究肥胖（obesity）的病因及肥胖基因（ob）的表达与调控．根据工就报道的h。b序列设计引物．经RT-PCR扩增中国人的山基因（包括信号抹在内的cDNA全长540bp）．PCR产物采用T－A克隆法首先连接到克隆载体PUC119．然后定向转移到经改造的真核表达载体PSV-β-lacZ，酶谱分析表明克隆基因为人的肥胖基因。
In order to better understand the pathoseny of the obesity and the mechanism of expres sion and manipulation of the obese (ob) gene- we designed and synthesized a pair of specific primers based on the published human ob gene to amplify Chinese ob gene by RT - PCR'The amplified prod uct, \'hich was 54obp in length including the whole DN A sequencescoding for the signal pep1ide and mature peptidc, was firstly 1igated to cloning vectorpU C119 and then directiona1ly transfered to modified eucaryotic expression vector pSV -6lacZ. Enzymatic analysis showed that the cloned gene was human obese gene
出处
《生物技术》
CAS
CSCD
1998年第4期9-12,共4页
Biotechnology
关键词
肥胖基因
克隆
真核表达载体
obese gene
cloning
eucaryotic expression vector
