小海马DPPH自由基清除作用及HPLC指纹图谱研究

Evaluation of DPPH Free Radical Scavenging Capability of Hippocampus japonicus Kaup. and Their HPLC Fingerprint

目的采用体外1,1-二苯基苦基苯肼(DPPH)抗氧化模型对海马提取物的抗氧化性质进行评价,并建立小海马HPLC特征指纹图谱,用于小海马药材的鉴别及质量评价。方法利用离线DPPH抗氧化评价体系对海马不同提取物进行评价,结果表明,海马水提物抗氧化能力最强,在此基础上又探明了海马水提物抗氧化能力随时间和浓度的变化规律,为海马抗氧化活性提供了科学依据。依据抗氧化活性实验结果,建立了海马水提物HPLC特征指纹图谱分析方法。结果海马水提物大部分化合物达到基线分离,方法的精密度、重现性、稳定性良好;建立小海马药材HPLC指纹图谱,采用中药指纹图谱相似度计算软件,对小海马进行真伪辨别和质量评价。结论表明该方法简捷、有效,是小海马药材鉴别及质量控制的有效方法。

OBJECTIVE To evaluate the antioxidant activity of the extracts of Haima, and to establish the HPLC fingerprint of Haima for the discrimination and the quality evaluation of Haima. METHODS Antioxidant properties of Haima extracts with different solvent were assayed in terms of antioxidant activity by scavenging activities on 1,1-diphenyl-2-picrylhdrazyl （DPPH）. The effect of time and concentration of Haima extract on the antioxidant activity was also studied. Secondly, a chromatographic fingerprint method was developed. RESULTS The antioxidant activity of water extract of Haima was higher than all the other extracts of Haima. The developed HPLC method was simple, accurate and reliable for the development of Haima fingerprint. Ten Haima samples collected from different medicine store were analyzed and the Haima HPLC fingerprint was established. The similarity of the HPLC chromatogram was performed for authentication and quality control of Haima. CONCLUSION The HPLC fingerprinting techniques have high potential in authentication or source-tracing types of applications.