免疫应激对不同品种仔猪免疫功能的影响

Effects of Immunological Stress on Immune Response in Different Breeds of Piglets

[目的]探讨免疫应激对莱芜猪、鲁莱猪及大约克夏断奶仔猪免疫功能的影响。[方法]分别选用12头体重为(13.6±0.5)kg的莱芜猪、鲁莱猪和大约克夏猪,采用2×3因子设计:免疫应激(注射LPS或生理盐水),品种类型(莱芜猪、鲁莱猪和大约克夏猪)。试验第1天,分别给每品种中的6头猪注射200μg/kg.BW的LPS,其余6头注射生理盐水作为对照,并进行猪瘟疫苗接种。注射LPS后的第2、7和14天,采血测定其淋巴细胞转化率;注射LPS前1天、注射LPS后第7天和第14天,测定其猪瘟抗体水平。[结果]猪在LPS刺激后的第2天,其淋巴细胞转化率显著高于生理盐水组(P<0.01),莱芜猪的淋巴细胞转化率显著高于大约克夏猪(P<0.05);免疫应激对不同品种仔猪的猪瘟抗体水平无差异性影响。[结论]LPS能够有效刺激不同品种猪的细胞免疫机能,并且不同品种仔猪免疫应答能力有差异。

[ Objective] The research aimed to explore effects of an immunological stress on immune response in different breeds of piglets （ Lu｜ai pig, Laiwu pig and Yorkshire ）. [ Method ] All the 12 weaning pigs （ Lulai, Laiwu and Yorkshire ） of （ 12.6 ± 0.5 ） kg were used in a 2×3 factorial design. The main factors consisted of immunological challenge （ LPS or saline） and breeds （ Lulai pig, Laiwu pig and Yorkshire ）. On day one ,6 piglets of each breed were injected with LPS at 200 μg/kg. BW or an equivalent amount of sterile saline, and injected swine plague vaccine at the same time. Blood sample were collected at 2,7 and 14 d post-injection to analysis the blood lymphocyte proliferation; The level of swine plague antibody of 1 d pro-injection and 7,14 d post-injection were test. [ Results ] On day 2 after injection, the lymphocyte transformation rate of piglets injected with LPS were significantly （P 〈 0.01 ） increased compared with piglets injected with saline. The lymphocyte transformation rate of Laiwu piglets were raised significant than Yorkshire piglets （P 〈 0.05 ）. Effects of immunological stress on the level of swine plague antibody had not significant difference in different breeds of piglets. [ Conclusion ] The results indicated that LPS can effectively stimulate cell immunity reaction in different breeds of piglets, and the immune response ability is different in various breeds of piglets.