人CD14基因的克隆及序列测定

Cloning and Sequencing of Human CD14 Gene

采用ＰＣＲ技术，从佛波酯乙醇刺激的ＨＬ６０细胞基因组ＤＮＡ中扩增获得了１１ｋｂ的人ＣＤ１４基因．序列分析表明，有四个碱基发生了突变，其中两处为首次报道．

Human CD14 gene was cloned from genomic DNA of PMAstimulated HL60 cells by PCR. Sequencing result showed that it is identical to the reported human CD14 gene except four bases, among which two new mutations were identified.