摘要
短串联重复序列（ＳＴＲ）是由几个碱基对作为核心单位串联重复形成的一类ＤＮＡ序列，应用３个ＳＴＲ基因座在同一反应体系中进行互不干扰的多重ＰＣＲ，采用高分辨力的聚丙烯酰胺凝胶电泳分离、银染法显影技术，对我国汉族的ＣＳＦ１ＰＯ，ＴＰＯＸ和ＴＨ０１等３个基因座等位基因的基因频率调查，ＣＳＦ１ＲＯ基因座，观察到９个等位基因，２２个基因型；ＴＰＯＸ基因座，观察到６个等位基因，１４个基因型：ＴＨ０１基因座，观察到６个等位基因，１９个基因型。获得了满意的结果，显示了广阔的应用前景。
The application of short tandem repeat (STR) polymorphisms has stimulated progress in the elaboration of linkage maps, forensic identification and paternity determination, characterization of disease genes, and simplification and precision of DNA typing. In this report. We have carried out DNA extraction from blood multiplex amplification of CSF1PO, TPOX and TH01. By using denaturing polyacrylamide gel electrophoresis and silver stain, we investigated the allele frequencies of CSF1PO, TPOX and TH01 loci in Han group living in Yunnan provice of China. In this study, 9 alleles and 22 genotypes of CSF1PO locus were observed; In the locus TPOX,6 alleles and 14 genotypes were observed. In the TH01 locus, 6 alleles and 19 genotypes were observed.
