摘要
介绍了单细胞微凝胶电泳(SCG)技术的原理和操作过程,并应用该技术研究了γ-线照射、过氧化氢(H_2O_2)、氯化镉(CdCl_2)对人血淋巴细胞DNA的损伤效应。结果表明,γ-线照射、H_2O_2和CdCl_2均能引起细胞DNA迁移长度增加,且呈显著的剂量效应关系。对未处理对照细胞DNA迁移的原因及SCG实验操作过程中应注意的事项也进行了讨论。
In this paper, the single cell microgel electrophoresis (SCG) technique wasdescribed in detail, and effects of DNA damage of human blood lymphocytes inducedby γ-rays radiation, H_2O_2 and CdCl_2 were studied using this technique. It was shownthat γ-rays, H_2O_2 and CdCl_2 all caused the increase of DNA migration length in thelymphocytes in the dose-dependent manner. The principle of the SCG techinque, causeof DNA migration in the untreated control cells, and noticeable details in the SCGtechnique were also discussed.
关键词
DNA损伤
淋巴细胞
电离辐射
SCG
H2O2
CDCL2
Single cell microgel electrophoresis
DNA damage
Lymphocyte
Ionizing radiation
H_2O_2
CdCl_2
