摘要
在栽培稻的籼粳亚种间,花药培养力存在显著差异,这一差异主要是由遗传因素引起的。以适合籼粳稻杂种花药培养的SK_3培养基,经花药培养获得了一个籼粳交F_1代的加倍单倍体(DH)群体,对该群体的110个株系用同一种培养基进行花药培养,利用该群体构建的分子图谱进行有关水稻花药培养力的数量性状基因座位(QTLs)的分析。结果表明,与水稻花药培养力有关的4个性状在DH群体中均表现为连续分布,愈伤组织诱导率与绿苗分化率之间不存在相关性,而绿苗产率与愈伤组织诱导率和绿苗分化率均显著相关。在第6、7、8、10和12 5条染色体上分别检测到与愈伤组织诱导率有关的5个QTLs,其加性效应均为正。在第1和第9染色体上检测到与绿苗分化率有关的2个QTLs,这两个性状间的QTs不存在连锁。在第9染色体上有一个主效基因与白苗分化率有关,对绿苗产率则没有检测到特有的QTL。
Anther culturability is significantly different between indica and japonica rice varieties. A doubled haploid (DH) population was produced on SK_3 medium suitable for anther culture of an indica/japonica hybrid. The anthers of 110 DH lines were plated on the above medium to analyze the quantitative trait loci (QTLs) for anther culturability using a molecular map constructed from this population. Continuous distributions were detected in four traits associated with the anther culturability. The frequencies of callus induction and green plantlet differentiation were not correlative, but both were strongly positive correlated to the frequency of green plantlet yield. For callus induction frequency, five QTLs were identified on chromosomes 6, 7, 8, 10 and 12 respectively, and two QTLs for green plantlet differentiation located on chromosomes 1 and 9. There was a major QTL for albino plantlet differentiation frequency on chromosome 9. No specific QTL was found in green plantlet yield frequency.
