摘要
建立了高效液相色谱法测定红糖中碱性橙含量的测定方法。采用C18分离柱,以乙腈和水[V（乙醇）∶V（水）=60∶40]为流动相,检测波长为443 nm。检出限为0.10 mg/kg,回收率为84.0%-107.0%,测定结果标准偏差2.0%-5.3%。
An analytical method for determination of Basic Brilliant Orange G in Brown Sugar by RP-HPLC was developed. The samples were extracted with ethanol. Basic Brilliant Orange G was determined by a reversed phase HPLC with C18 column using UV detection at 443 nm. The results showed the linearity range of this method was 0.05 -4.00 mg/L for Basic Brilliant Orange G. The recovery rate of Basic Brilliant Orange G was in the range of 84.0% to 107.0% with less than 5.3% of coefficient of variation.
出处
《云南化工》
CAS
2009年第5期24-26,共3页
Yunnan Chemical Technology
关键词
红糖
HPLC法
碱性橙
Brown Sugar
HPLC
Basic Brilliant Orange G
