野油菜黄单胞菌野油菜致病型胞外多糖合成有关的DNA序列的克隆

CLONING OF DNA SEQUENCES INVOLVED IN EXOPOLYSACCHARIDE SYNTHESIS OF XANTHOMONAS CAMPESTRIS PV.CAMPESTRIS

以从野油菜黄单胞菌野油菜致病型（Xanthomonascampestrispv.campestris）胞外多糖突变体T117中克隆到的含有突变序列的DNA片段作探针，从野生型菌株中鉴定和克隆了含有相应序列的9．4kbHindⅢ片段。该片段可反式互补突变体T117，完全恢复其胞外多糖的合成，说明该片段至少含有一个完整的与该菌胞外多糖合成有关的基因。

By using a cloned DNA segment containing the mutated sequences from the exopolysaccharide-deficient mutant T117 of Xanthomonas campestris pv. campestris as a probe, a 9.4kb HindⅢ fragment harbouring DNA sequences corresponding to the mutated site was identified and cloned from the wild-type strain. The fragment could complement the mutant strain T117 in trans and restore the synthesis of exopolysaccharide. TIs indicates that the fragment contains at least an intact gene which is involved in the exopolysaccharide synthesis of Xanthomonas campestris pv.campestris.