斑点热群立克次体的PCR/SSCP分型技术研究

GENOTYPIC IDENTIFICATION OF SPOTTED FEVER GROUP RICKETTSIAE BY PCR AND SSCP ASSARY

本文应用PCR／SSCP技术对斑点热群立克次体国际标准株、国内各参考株及黑龙江立克农作54株、36株进行了分型鉴定。结果显示各株之间SSCP图谱差异明显，国内各参考株的SSCP，图谱与西伯利亚立克次体的图谱完全一致，黑龙江立克次作的图谱与国内各株显著不同。分析表明，我国存在2种斑点热群立克次体，即西伯利亚立克农作种和斑点热群立克次体新种－黑龙江立克次体。同时证明应用PCP／SSCP技术分析斑点热群立克农作型别，具有方法简单、经济省时、结果准确等优点。该分型技术应用于立克次体分型国内外未见报道。

CR and SSCP method was used to analysis the gendric type of fockettsial strains iso-lated from China and the international reference strains. The results indicated that therewere two strains of spoted fever group rickettsiae, R. sibria and R. heilongjiang, in china, andtheir SSCP patterns were different obviously- Our studies domonstrated that the PCR andSSCP method for the identification of spotted fever group rickettsiae was suitable for routinerecognition of isolates of rickcttsial species and genotypes.