大肠杆菌发酵重组人白细胞介素-2的高密度表达

PRODUCTION OF RECOMBINANT IL-2 BY E.COLI WITH HIGH CELL DENSITY AND HIGH EXPRESSION LEVEL

利用美国NBS公司BibFloⅢ型发酵罐，采用连续流加的方法培养能表达IL-2的E．coli，结果细菌温重达100g／L发酵液，IL－2活性达1．52×1010U／L发酵液。

ecombinant E. coli JM1O3 was used to produce recombinant human interleukin- 2 ina 5L BiofloⅢ fermentor(NBS, Newiersy, USA). In the nutrient feeding process,cell densityof l00g wet weight/L media and IL - 2 activity of l. 52 X 1O10U/L media were obtained.