柯萨奇B_4病毒在体外对小鼠胰岛素分泌功能的影响

EFFECT OF SECRETIVE FUNCTION OF INSULIN IN MICE INFECTED WITH COXSACKIEVIRUS B_4 IN VITRO

柯萨奇B4病毒（CB4V）感染被认为和胰岛素依赖型糖尿病（IDDM）的发病有关。我们对体外培养的小巴胰岛和胰岛细胞经CB4V感染后，检测其胰岛素分泌功能。结果显示，病毒感染组的胰岛包膜的消失较对照组早；糖刺激的胰岛素释放量明显低于对照组，但CB4V并不引起胰岛细胞的溶解。这表明：（1）CB4V可在体外感染小鼠胰岛和胰岛细胞。（2）CB4V可损害胰岛β－细胞合成胰岛素的功能。

Infections wth Coxsackie viruses(especially coxsakie B4 ) are thought to be involved inthe pathognesis of insulin - dependent diabetes mellitus(IDDM). Mouse pancreatic ialets andβ - cell cultured in vitro were infected with coxsakie B4 virus(CB4V), the effects of viruses onthe islets and β - cell were assessed by examination of their secreted functions of insulin. Itwas found that the islets envelope infected with CB4V disapperared earlier and the(pro)in-sulin conent of ialets at stimulatory gluare conentrations is low sighficantly, comparedwith control groups. But did not cause any β - cell destruction. It was suggested that (1 )CB4V can infected mouse pancreatic islets and/orβ - cell in vitro. (2)That infection withCB4V for islets and β - cell leads. to an impairment of insulin metahalic functtions of β - cells.