摘要
利用紫外-可见吸收光谱和荧光光谱研究了黄酮类化合物山奈酚与小牛胸腺DNA（ct-DNA）之间的相互作用。随着DNA的加入,山奈酚的吸收光谱逐渐发生明显的红移和减色效应,通过双倒数法拟合分析,得到山奈酚与ct-DNA的结合常数为1.2×10^4。山奈酚自身荧光很弱,与DNA结合后其荧光明显增强,表明山奈酚可以作为荧光探针来开展与生物大分子相互作用的研究。
The interaction between kaempferol and calf thymus DNA（ct-DNA） was studied by UV-Visible absorption and fluorescence spectroscopies. With the increasing amount of ct-DNA added to kaempferol,obvious red shift and hypochromic effect were observed in the UV-Visible absorption spectra of kaempferol. The binding constant between kaempferol and ct-DNA was obtained as 1.2×10^-4 by double-reciprocal analysis. Compared to the weak fluorescence of kaempferol, the fluorescence intensity was evidently enhanced with the combination to ct-DNA, indicating that kaempferol could be used as a fluorescence probe to investigate the interactions with biomacromolecules.
出处
《光谱实验室》
CAS
CSCD
北大核心
2009年第5期1211-1214,共4页
Chinese Journal of Spectroscopy Laboratory
基金
国家自然科学基金(No.20772011)资助项目
