HPLC测定自制胃粘附制剂在大鼠胃内的组织残留量

Determination of Tinidazole in Rat's Gastric Ulcer by HPLC

采用Diamonsil-C18色谱柱(200×4.6mm,5μm),甲醇-水(20:80)为流动相,甲硝唑为内标,紫外检测波长317nm,流速1.0mL/min,柱温30℃,高效液相色谱法(HPLC)测定自制替硝唑胃粘附制剂在SD大鼠胃内的组织残留量。结果表明,替硝唑浓度在0.5-50mg·L^(-1)范围内与替硝唑峰面积和甲硝唑峰面积之比线性关系良好(r=0.9998),检出限为0.15mg·L^(-1),相对标准偏差小于6.7%,平均回收率为94.0%—102.6%。方法简便,准确可靠,适用于生物组织样品中替硝唑的测定。

Residue level of tinidazole in rat s stomach was determined by HPLC with Diamonsil-C18 column（200×4.6mm,5μm）,mobile phase of methanol-H_2O（20：80）at 317nm.The linear range of tinidazole was 0.5—50mg·L^-1（r=0.9998） with the average recovery of 94.0%—102.6%.This method is simple,credible and available for the assay of tinidazole in biological specimen.