摘要
目的探讨成纤维细胞内的iNOS活性的调控机制。方法以MTT比色法检测细菌脂多糖(LPS)处理后NIH3T3细胞的生长曲线,以Greiss法检测LPS处理NIH3T3细胞一定时间后培养液中NO2-含量。结果低浓度LPS对NIH3T3细胞增殖有促进作用,产生的NOt呈LPS浓度依赖性并依赖于培养液中的血清,高浓度(>1000ng/ml)LPS可抑NIH3T3细胞增殖且产生NO2-不依赖于培养液中的血清。结论LPS可通过血清依赖或非血清依赖两种方式增强成纤维细胞iNOS活性,对细胞增殖则有双重作用。
Aim To study mechanism of activation of inducible NO synthase (iNOS) of fibroblast. Methods determining the growth curve of NIH 3T3 cells treated with LPS using MTT colorimeteric assay, and detecting the NO2- in culture medium using Greiss' assay after the cells incubated with LPS for a given period. Results Low-dose LPS enhanced the growth of NIH 3T3 cells, NO2- induced was LPS-dose dependent and serumdependent, however, high-dose LPS (>1000ng/ml) inhibited the growth of the cells, NO2- induced was serumindependent. Conclusions LPS can enhance the iNOS activity of fibroblast in serum-dependent or serum-independent manner, LPS has a dual effect on growth and prolifertion of fibroblast.
出处
《中国人兽共患病杂志》
CAS
CSCD
北大核心
1998年第6期19-22,共4页
Chinese Journal of Zoonoses