双歧杆菌完整肽聚糖对小鼠骨髓来源树突状细胞分泌IL-12的影响

The influence of whole peptidoglycan of Bifidobacterium on interleukin-12 produced by dendritic cells derived from bone marrow of mice in vitro

目的探讨相同剂量双歧杆菌完整肽聚糖(WPG)在不同时间对小鼠骨髓来源树突状细胞分泌IL-12的影响。方法将双歧杆菌WPG与小鼠骨髓来源树突状细胞共培养,分别在加入WPG后0、12、24、36、48和60h收集培养上清液,用ELISA法测定不同时间点上清液中IL-12的量。结果双歧杆菌WPG与树突状细胞共培养后,上清液中IL-12的量在24h内逐渐升高,至24h达高峰,24h后呈下降趋势;同0h组比较,12、24和36h组IL-12分泌量明显增加(P<0.05),而48、60h组同0h组比较差异无显著性(P>0.05)。结论双歧杆菌WPG能够刺激小鼠骨髓来源的树突状细胞分泌IL-12;双歧杆菌WPG对树突状细胞的免疫刺激存在时间效应关系。

Objective To explore in vitro the effect of whole peptidoglyean of Bifidobacterium （WPG） in a same dose on the interleukin 12 secretion by the DCs deriving from bone marrow at different time. Method In vitro, immature DCs were induced by monocytes cultured in granulocytes macrophage-colony stimulating factor（GM-CSF） and interleukin-4 on the 7th day and then exposed to WPG（5 μg/ml）. The supernatant was collected at 0 hour,12 hour,24 hour,36 hour,48 hour and 60 hour respectively after exposing to WPG, and the level of interleukin 12 was detected by Enzyme-labeled immunosorbent assay （ELISA）. Result The level of interleukin-12 secreted by dendritic cells co-cultured with WIG increased gradually in 24 hours, reached the peak at 24 hour and then decreased 24 hours later. Compared with the 0 hour group, the levels of IL-12 in the 12 hour group,the 24 hour group and the 36 hour group were significantly higher （P 〈0.05）, while neither the 48 hour group nor the 60 hour group were significantly different from the 0 hour group （P 〉 0.05）. Conclusion The level of IL-12 secreted by DCs derived from bone marrow had been remarkably elevated under the stimulation of WIG. Meanwhile the immunostimulation of WIG had time-effect relationship.