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PCR-DGGE技术在动物肠道微生态研究中的应用 被引量：4

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摘要 本文综述了PCR-变性梯度凝胶电泳(PCR-DGGE)分析微生物多态性的原理,还介绍了它的技术步骤、在动物肠道微生态研究中的主要应用以及优缺点,为更好的研究动物肠道微生物奠定了基础。
作者 周小娟 张日俊
机构地区 中国农业大学饲料生物技术动物营养学国家重点实验室
出处 《中国微生态学杂志》 CAS CSCD 2009年第10期956-957,共2页 Chinese Journal of Microecology
关键词 PCR-DGGE 原理 应用
分类号 R378 [医药卫生—病原生物学]
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参考文献12

  • 1倪学勤,Joshua Gong,Hai Yu,曾东,Shayan Sharif,周小秋.采用PCR-DGGE技术分析蛋鸡肠道细菌种群结构及多样性[J].畜牧兽医学报,2008,39(7):955-961. 被引量：56
  • 2徐敏娟.鹅肠道细菌多样性的分子生态学初步研究[P].扬州大学硕士学位论文.
  • 3朱伟云,姚文,毛胜勇.变性梯度凝胶电泳法研究断奶仔猪粪样细菌区系变化[J].微生物学报,2003,43(4):503-508. 被引量：81
  • 4姚文,朱伟云,毛胜勇.16S rDNA技术研究新生腹泻仔猪粪样细菌区系的多样性变化[J].微生物学报,2006,46(1):150-153. 被引量：28
  • 5姚文,朱伟云,韩正康,Antoon D L Akkermans,Barbara Williams,Seerp Tamminga.应用变性梯度凝胶电泳和16SrDNA序列分析对山羊瘤胃细菌多样性的研究[J].中国农业科学,2004,37(9):1374-1378. 被引量：53
  • 6COLLIER C T,SMIRICKY-TJARDES M R,ALBIN D M,et al. Molecular ecological analysis of porcine ileal microbiota responses to an timicrobial growth promoters[ J]. J Animal Sci ,2003,81:3035-3045.
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二级参考文献61

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共引文献176

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二级引证文献8

中国微生态学杂志
2009年 第10期

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