大鼠肝大部切除后脾对其再生能力的影响

Role of spleen on hepatic regeneration after partial hepatectomy

目的：探讨脾脏在肝大部分切除后对其再生能力的影响。方法：将５０只ＳＤ大白鼠随机分成两组。Ａ组：行脾切除加７０％肝大部分切除；Ｂ组：模拟脾切除加７０％肝大部分切除。结果：在肝大部分切除后第１天和第２天，Ａ组肝再生指数分别为２．６３％±０．２９％和３．５１％±０．２２％，Ｂ组分别为１．６５％±０．２７％和２．６２％±０．３２％，Ａ组明显重于Ｂ组（Ｐ＜０．０５）。血清转氨酶：Ｂ组术后第１天９７２．４７±１８９．２５μｍｏｌ／Ｌ，明显高于Ａ组１０７．０５±５７．８９μｍｏｌ／Ｌ（Ｐ＜０．００５）。肝细胞核有丝分裂指数及肝细胞ＤＮＡ增殖抗原指数术后第１天Ａ组分别为３９．７／１０００±７．６／１０００肝细胞、１７．２％±０．５４％；Ｂ组分别为９．８４／１０００±１．２／１０００肝细胞、５．２％±０．２３％（Ｐ＜０．０５和Ｐ＜０．００１）。结论：脾脏在肝大部分切除后，对肝再生起着十分重要作用。可能是脾脏在肝大部分切除后介导炎性反应而抑制肝脏再生。

Objective:To evaluate the role of spleen in hepatic regeneration after partial hepatectomy.Methods:50 female SD rats were divided at random into two groups:group A consisted of rats undergone splenectomy and partial hepatectomy;group B consisted of rats undergone shamed operation and partial hepatectomy.Results:24 and 48 hours after partial hepatectomy the weights of the liver in group A(2.63%±0.29% and 3.51%± 0.22 %) were significantly heavier than those in group B(1.65%±0.27% and 2.62%±0.32%)( P <0.05).The ALT of group B(972.47±189.25 μmol/L) 24 hours after operation was significantly higher than that of group A ( 107.05 ±57.89 μmol/L)( P <0.005).The mitotic index and DNA proliferative antigen index of hepatic cells in groups A (39.7/1 000±7.6/1 000 hepatic cells and 17.2%±0.54%) 48 hours after operation were significantly higher than those of group B (9.84/1 000±1.2/1 000 hepatic cells and 5.2%±0.23%)( P < 0.05 , P <0.001).Conclusions:The results show that the spleen plays an inhibitory role in hepatic regeneration after partial hepatectomy.It is possible that spleen can induce an inflammatory damage influencing hepatic regeneration after partial hepatectomy in rats.