糖尿病大鼠周围神经IGF-1及VEGF的动态表达及巴曲酶对其的影响

The dynamic expression of IGF-1 and VEGF and the impact of batroxobin on it in peripheral nerve of diabetic rats

目的探讨胰岛素样生长因子(IGF-1)及血管内皮生长因子(VEGF)在糖尿病周围神经病(DPN)大鼠中的动态变化规律及巴曲酶对IGF-1和VEGF的影响并探讨其机制,为临床治疗DPN提供理论依据。方法大鼠腹腔注射链脲佐菌素制作实验性糖尿病模型,分别于成模后2m和3m时腹腔注射巴曲酶进行干预。通过免疫组织化学和原位杂交双重检测IGF-1及VEGF在坐骨神经中的表达。结果DM造模后2m时坐骨神经中IGF-1及VEGF的含量均明显减少,且随时间变化有统计学差异,而巴曲酶治疗后IGF-1的表达无明显改变,VEGF的表达明显增加。结论:DPN大鼠坐骨神经中IGF-1及VEGF表达减少可能参与DPN的发病机制,而巴曲酶对DPN的保护作用,并非通过对IGF-1的调节,其机制可能包括对VEGF的调节。

Objective To investigate the dynamic regularity of IGF-1 and VEGF in diabetic peripheral neuropathy （DPN） rats and to confirm the impact of batroxobin on IGF-1 and VEGF tentatively and the mechanism in order to furnish theorial foundation for the therapy of DPN clinically. Methods Through the peritoneal injection of streptozoein to make the experimental diabetes models ,we injected batroxobin into abdominal cavity of rats differently 2 months and 3 months after the formation of DM model for intervention. Immunohistochemistry and hybridization in situ were used to detect thd expression of IGF-1 and VEGF in sciatic nerve of the rats. Results The content of IGF-1 and VEGF in sciatic nerve decreased obviously 2 months after the formation of DM model and it had time-varying statistics diversity, however the expres- sion of IGF-I did not alter markedly, and the expression of VEGF was markedly increased after the treatment of batroxobin. Conclusion The decreased expression of IGF-1 and VEGF in sciatic nerve in DPN rats might play an important role in the pathogenesis of DPN. Treatment with batroxobin may be beneficial in the prevention of DPN,but it has no relationship with the regulation of the expression level of IGF-1, and possibly with the regulation of the expression level of VEGF in the sciatic nerves of the DPN rats.