4℃储存条件下不同组织保存液对皮片活力影响的研究

Effect of various methods of tissue storage on split skin viability at 4℃

目的探讨不同组织器官保存液在４℃条件下储存对皮片活力的影响。方法在４℃储存条件下，采用普通培养液（ＭＥＭ）、肝肾等组织保存液（ＵＷ）和ＭＥＭ加硫酸软骨素（ＣＳ），以及ＵＷ换液（ＵＷＲ）、ＭＥＭ换液（ＭＥＭＲ）５个实验组，储存猪皮片，在储存１天、３天、５天、７天、１０天和１４天共６个时间点内分别采用新型四氮唑盐法测定皮片活力值。结果３种保存液对皮片活力均具有保护作用，尤以ＵＷ组优于ＭＥＭ组，定期换液的ＵＷＲ组及ＭＥＭＲ组效果更优于相同条件的不换液组ＵＷ组及ＭＥＭ组。

Objective To study various tissues and organs storage solutions (liver,kidney and corneal) at 4℃ for the storage of skin and the viability of skins stored in these solutions at correspondent condition. Method Five experiment groups investigated for storing porcine split skin at 4℃ were MEM,UW,MEM plus CS,UW R and MEM R.A new assay,WST 1, was applied to evaluate skin viability at 1,3,5,7,10 and 14 day during storage respectively. Result Skin viability can all be protected by these three solutions.The effect of UW solution was superior to MEM.UW R and MEM R were superior to MEM and UW. Conclusion The method of UW R which replaces medium routinely may be an optimal method of storing skin at 4℃.