柔红霉素对K562细胞凋亡的影响

Influence of daunorubicin on apoptosis factor of K562 cells

目的研究柔红霉素(DNR)对白血病K562细胞细胞凋亡的影响。方法对数生长期K562细胞随机分A、B两组,A组(对照组):加RPM I-1640培养液;B组(DNR组):分别加DNR0.2、2、20μmol/L分别作用1、2、6、24 h后,用流式细胞检测术检测K562细胞的凋亡率。结果在没有药物的作用下,K562细胞存在自然凋亡。DNR组:0.2μmol/L分别作用1、2、6、24 h,细胞凋亡率与对照组相比差异均无统计学意义(P>0.05);2μmol/L作用于K562细胞1 h,与对照组相比差异无统计学意义(P>0.05);作用2、6、24 h,与对照组相比差异均有统计学意义(P<0.01);20μmol/L作用1、2、6、24 h,与对照组相比差异均有统计学意义(P<0.01)。结论DNR能促进K562细胞凋亡,并存在剂量、时间依赖性。

Objective To explore the influence of daunorubicin on apoptosis factor of K562 cells. Methods The K562 cell was divided into control group and DNA group. Control group： the K562 cells were respectively cultivated with RPMI - 1640; daunombiein（DNA） group：the I（562 cells were treated respectively with respectively of DNR 0.2,2 and 20 μmoL/L. The apoptosis factor of K562 cells was determined by FCM,after respectively treated for 1,2,6 and 24 h. Results K562 cells existed natural apoptosis without durg treatment. After treated for different time with DNA 0.2 μmol/L,the rates of apoptosis fac, tor of K562 c, ells in DNA group had no significant difference compared with control group （ P 〉 0.05）. After treated for 1 h with DNA 2 μmol/L,there was no significant difference compared to the contrast group（ P 〉 0.05 ）. After treated for 2,6 and 24 h, DNA 2 μmol/L, there was significant difference compared with the control group（ P 〈0.01 ）. Treatment for 1,2,6 and 24 h with DNA 20 μmol/L,the apoptosis rates were obviously difference compared with control group （ P 〈 0. 01 ）. Conclusion DNR can induce the apoptosis of K562 cell, which existed dose - dependence and time - dependence for the effect of DNR.