期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

HHcy大鼠学习记忆能力和基底前脑ChAT的变化及早期叶酸干预 被引量：1

下载PDF
导出
摘要 目的观察高同型半胱氨酸血症(HHcy)对大鼠学习记忆能力和基底前脑胆碱乙酰基转移酶(ChAT)的影响及早期叶酸(FA)干预的作用。方法将Wistar大鼠随机分为对照组、HHcy组和FA组,对照组给予饮用水,在HHcy组饮用水中添加蛋氨酸1.0g/(kg·d),在FA组饮用水中添加蛋氨酸1.0g/(kg·d)并予FA0.4mg/(kg·d)灌胃,共进行8周,测定各组血浆同型半胱氨酸(Hcy)、FA的基础浓度及实验满8周时的浓度,Morris水迷宫实验评估各组的学习记忆能力,并对大鼠基底前脑进行ChAT的免疫组化实验,对其灰度值进行分析。结果HHcy组的学习记忆能力较对照组和FA组均减退(P<0.01),经灰度分析比较后显示HHcy组基底前脑隔内侧核、Meynert’s基底核ChAT阳性细胞的表达与其余两组比较差异有显著性(P<0.01)。结论过量的蛋氨酸饮食可致HHcy,影响学习记忆能力,HHcy影响学习记忆能力考虑与基底前脑核团的胆碱能神经元的减少有关;早期补充叶酸可降低血浆Hcy水平,改善HHcy对学习记忆能力的影响。
作者 王延平 刘湘敏 陈盛强 黄绍宽 杨少青 孙卫文 李海员
机构地区 广州医学院第二附属医院神经内科 广东省广州市花都区人民医院神经内科 广东省广州市老人院
出处 《广东医学》 CAS CSCD 北大核心 2009年第11期1624-1627,共4页 Guangdong Medical Journal
基金 广东省科技计划项目(编号:2006B35602014)
关键词 高同型半胱氨酸血症 叶酸 学习记忆能力 基底前脑 胆碱乙酰基转移酶
分类号 R-332 [医药卫生] R285.5 [医药卫生—中药学]
  • 相关文献

参考文献10

  • 1BAYDAS G, OZER M, YASAR A, et al. Melatonin improves learning and memory performances impaired by hyperhomocysteinemia in rats[J]. Brain Res, 2005, 1 046(1 -2) : 187 -194.
  • 2GILANI G S, ROBERT W P, HERBERT G B. Effects of folic acid, vitamin B - 12 and vitamin B -6 supplementation on methionine- induced hyperhomocysteinemia in rats [ J ]. Nutrition Research, 2001, 21 : 1501 - 1 507.
  • 3ALGAIDI S A, CHRISTIE L A, JENKINSON A M, et al. Long - term homocysteine exposure induces aherations in spatial learning, hippocampal signalling and synaptic plasticity [ J]. Exp Neurol, 2006, 197(1) : 8 -21.
  • 4VOGELS O J, BROERE C A, TER LAAK H J, et al. Cell loss and shrinkage in the nucleus has,is Meynert complex in Alzheimer' s disease [ J ]. Neurobiol Aging, 1990, 11 ( 1 ) : 3 - 13.
  • 5SUNDEN S, RENDUCHINTALA M, PARK E, et al. Betaine - homocysteine methyhransferase expression in porcine and human tissues and chromosomal localization of the human gene[ J ]. Arch Biochem Biophys, 1997, 345( 1 ) : 171 - 174.
  • 6HOLM P I, BLEIE O, UELAND P M, et al. Betaine as a determinant of postmethionine load total plasma homocysteine before and after B - vitamin supplementation [ J ]. Arterioscler Thromb Vase Biol, 2004, 24(2) : 301 -307.
  • 7Institute of Medicine, National Academy of Sciences USA. Dietary reference intakes for folic acid, thiamin, riboflavin, niacin, vitamin B - 12, panthothenic acid, biotin, and choline[ M]. Washington, DC: National Academy Press, 1998.
  • 8BUCHMAN A L, AMENT M E, SOHEL M, et al. Choline deficiency causes reversible hepatic abnormalities in patients receiving parenteral nutrition: proof of a human choline requirement: a placebo - controlled trial [ J ]. J Parenter Enteral Nutr, 2001,25 ( 5 ) : 260 - 268.
  • 9DA COSTA K A, GAFFNEY C E, FISCHER L M, et al. Choline deficiency in mice and humans is associated with increased plasma homucysteine concentration after a methionine load [ J]. Am J Clin Nutr, 2005, 81(2): 440-444.
  • 10HOP I, ASHLINE D, DHITAVAT S, et al. Folate deprivation induces neurodegeneration : roles of oxidative stress and increased homocysteine [ J]. Neurobiol Dis, 2003, 14 ( 1 ) : 32 - 42.

同被引文献15

  • 1彭海,李方明,叶妮.维生素B_(12)影响同型半胱氨酸对大鼠认知能力的作用[J].营养学报,2005,27(2):101-104. 被引量：3
  • 2Seshadri S,Beiser A,Selhub J,et al.Plasma homocysteine as a risk factor for dementia and Alzheimer's disease[J].N Engl J Med,2002,346:476-483.
  • 3Tarkun I,Cetinarslan B,Canturk Z,et al.Homocysteine concentrations in type 2 diabetic patients with silent myocardial ischemia:a predictive marker[J].J Diabetes Complications,2004,18:165-168.
  • 4Peila R,Rodriguez BL,Launer LJ.Type 2 diabetes,APROE gene and the risk for dementia and related pathologies:The Honolulu Asia Aging Study[J].Diabetes,2002,51:1256-1262.
  • 5Whitmer RA.Type 2 diabetes and risk of cognitive impairment and dementia[J].Cur Neurol Neurosci Rep,2007,7:373-380.
  • 6Sasaki N,Fukatsu R,Tsuzuki K,et al.Advanced glycation end products in Alzheimer's disease and other neurodegenerative diseases[J].Am J Pathol,1998,153:1149-1155.
  • 7Hofmann MA,Kohl B,Zumbach MS,et al.Hyperhomocyst(e)inemia and endothelial dysfunction in IDDM[J].Diabetes Care,1997,20:1880-1886.
  • 8Makris M.Hyperhomocysteinemia and thrombosis[J] Clin Lab Haematol,2000,22:133-134.
  • 9De la Torre JC.Alzheimer's disease is a vasocognopathy:a new term to describe its nature[J].Neurol Res,2004,26:517-524.
  • 10Bailey TL,Rivara CB,Rocher AB,et al.The nature and effects of cortical microvascular pathology in aging and Alzheimer's disease[J].Neurol Res,2004,26:573-578.

引证文献1

二级引证文献2

广东医学
2009年 第11期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部