摘要
介绍萃取-沉淀法提取分离诃子多酚及利用红外光谱法对诃子多酚进行结构鉴定和利用Fenton反应产生羟自由基·OH,光照核黄素产生超氧阴离子自由基O2-·,分光光度法研究诃子体外清除活性氧自由基的作用。用硫代巴比妥酸(TBA)分光光度法研究诃子对·OH诱发卵磷脂脂质过氧化损伤的抑制作用。试验结果表明诃子能有效清除活性氧自由基。
Extraction-precipitation methods used in separation of terminalia-polyphenols and structures of terminalia-polyphenols by IR spectra. Scavenging effects of terminalia on active oxygen species · OH and O2^-· generated by Fenton reaction and riboflavine photosensitization in vitro were investigated by the means of spectrophotometry. The inhibition effects of terminalia on the lecithin lipid peroxidation and oxidation damage of DNA chain induced by hydroxyl radical were observed by thiobarbit uric acid spectrophotometry. The results showed that terminalia could scavenge active oxygen efficiently. It could also significantly inhibitlipid peroxidation and oxidation damage of DNA by hydroxyl radical.
出处
《食品研究与开发》
CAS
北大核心
2009年第11期6-9,共4页
Food Research and Development
基金
云南省教育厅科学研究基金项目(2007B148M)
关键词
诃子
多酚
分离
纯化
抗氧化
terminalia
polyphenols
isolation
purification
antioxidant ability
