摘要
目的:研究免疫八肽的纯化工艺。方法:以水-乙腈为二元梯度洗脱流动相,以0.1%三氟乙酸(TFA)为离子对,用RP-HPLC(反相高效液相色谱)对合成产物进行分析,探索主峰出峰时间和杂肽分离度,优化程序后将程序放大并制备纯化,用RP-HPLC分析纯肽产物并与标准品对照其色谱保留值,结果一致,结果:此程序下收集的纯肽产物是目标八肽,并且收集的3段肽液纯度全部大于95%,产率高,纯度高。结论:建立了适于大规模生产免疫八肽(AT-N)的纯化工艺。
Objective： The purification crafts of Immunity eight peptide was studied. Methods： Took water-methyl cyanide as dual gradient elution flowing and 0.1% TFA as the granules. Analyse the purification of the product by RP-HPLC, to detecte the peak time of the prominent peak and the degree of dissociation of mixed peptide. After optimizing the procedure, enlarged the procedure and the prepared and the purified it, analysized the pure peptide product by RP-HPLC and compared its chromatograph retention value with the stan- dard, the result was consistent. Results： The pure peptide product which collected under this procedure was the goal peptides ,and the purity of the peptide fluid collected in the three sections was bigger than 95%. Conclusions： The production and the purity rate was high. The purification crafts is suitable for the large scale production of immunity eight peptidcs（ AT-N ）.
出处
《现代生物医学进展》
CAS
2009年第18期3455-3460,共6页
Progress in Modern Biomedicine
关键词
分离度
梯度洗脱
色谱保留值
Degree of dissociation
Gradient elution
Chromatograph retention value
