葛根素对腹膜透析液诱导人腹膜间皮细胞分泌TGF-β_1的影响

Effect of Puerarin on the secretion of TGF-β_1 by HPMC cultured in PDS

目的:了解葛根素对腹膜透析液干预下的体外培养人腹膜间皮细胞分泌转化生长因子β(1TGF-β1)的影响。方法:体外培养的人腹膜间皮细胞,分对照组、PDS组、A组(葛根素终浓度为50μg/mL)、B组(葛根素终浓度为100μg/mL)、C组(葛根素终浓度为200μg/mL)五组观察。检测各组上清液中TGF-β1的含量并比较。结果:腹膜间皮细胞在PDS干预下,TGF-β1分泌显著增加,添加葛根素再干预的A、B、C组TGF-β1分泌与PDS组比较有显著下降。结论:葛根素对腹膜透析液诱导的腹膜间皮细胞分泌致纤维化因子TGF-β1具有抑制作用。

Objective： To investigate the effect of Puerarin on the secretion of TGF- β1 of human peritoneal mesothelial cell （HPMC） cultured with PDS. Methods： Human peritoneal mesothelial cells （HPMC） were cultured in vitro and divided into five groups （RPMI1640 culture medium as control, PDS containing 2.5% glucose as PDS group, PDS containing 2.5% glucose and 100μg/mL, 200μg/mL or 400μg/mL Puerarin as group A, group B and group C respectively）. Aider 48-hour incubating,the levels of TGF-β1 in supernatant were detected and compared. Results： Significant lower level of TGF-β1 in supernatant were found in control group, as well as in group A,B and C, when compared with that in PDS group （P〈0.01）. Conclusion： Puerarin reduce the secretion of YGF-β1 induced by commercial PDS in cultured human peritoneal mesothelial cell.