蛹虫草栽培基质的模型优化研究

Optimization on mathematical model of basic medium of Cordyceps militaris cultivation

【目的】对蛹虫草人工栽培的栽培基质进行优化。【方法】以蛹虫草菌株CDM-003为供试材料,采用400mL玻璃罐头瓶为栽培容器,研究了蚕蛹粉、基质含水率、葡萄糖、蛋白胨和微量元素添加剂对蛹虫草子座生物学效率的影响。通过建立数学模型,确定蛹虫草人工栽培中上述几种常用基本基质用量与子座生物学效率之间的函数关系。【结果】确定了基质中各主要成分的最佳用量为:蚕蛹粉0.156g/g,葡萄糖12.19g/L,蛋白胨10g/L,基质含水率66.75%,微量元素添加剂25g/L。【结论】蚕蛹粉用量、基质含水率及栽培营养液中葡萄糖、蛋白胨、微量元素添加剂的含量对蛹虫草的生物学效率具有显著的影响。

[Objective] The study was to optimize the cultivated medium of Cordyceps militaris. [Method] The effects of silkworm chrysalis powder, glucose, peptone, water and trace element additive on stroma biological efficiency of Cordyceps militaris were studied with Cordyceps militaris CDM-003 as the test material, which was cultivated in 400 mL cans in this study. The functional relationship between these several kinds of common medium components and the stroma biological efficiency was fitted by mathematic model. [Result] The optimum medium was： 0. 156 g/g silkworm chrysalis powder, 12.19 g/L glucose, 10 g/L peptone, 66. 75 % water and 25 g/L trace element additive. [Conclusion] The effects of silkworm chrysalis powder, glucose, peptone, water and trace element additive on stroma biological efficiency of Cordyceps militaris were significant and the key to increase the yield of stroma of Cordyceps militaris was the optimum combination of all the above factors.