摘要
细胞或质体中部正确分裂位点的选择是MinD蛋白与其他Min蛋白(MinC/E)相互作用的结果,MinD蛋白在原核细胞以及植物叶绿体的分裂过程中发挥着重要的作用。细胞中MinD蛋白浓度的明显升高可影响正常细胞的分裂过程而产生丝状体细胞。为了研究叶绿体分裂蛋白CrMinD的保守功能,构建了衣藻CrMinD-gfp的原核表达重组质粒进行了原核功能验证。试验结果表明,衣藻CrMinD蛋白的过量表达严重影响了大肠杆菌的分裂,其在原核细胞中运动和定位与用GFP标记的原核细胞MinD蛋白具有相似性。更进一步证明了叶绿体分裂同源物CrMinD蛋白与原核细胞MinD蛋白有着相似的功能,是一个进化上功能保守的蛋白。同时,这一结果也为研究植物细胞中质体的分裂机制奠定了一定的基础。
The interacting of MinD and MinC and MinE proteins of Escherichia coli are required for proper placement of the division septum at midcell, The MinD protein plays a key role in the course of bacterial cell and plant chloroplast division. The over production of MinD protein can block the normal cell division and lead to a filamentation cell. In plant cells, There are clear similarities between bacterial and plastid division, but limited information exists regarding the mechanism of plastid division in higher plants. Here we constructed a expression plasmid encoding the full-length CrMinD, an Chlamydomonas reinhardtii homologue of the bacterial MinD. In agreement with cell division studies in bacteria over expression of CrMinD in E. coli results in filamentation cell formation. The sesuggestion that CrMinD is an evolutionary conserved cell division protein. Meanwhile, the result lays a certain foundation for studying on the division mechanism of plant chloroplast.
出处
《云南植物研究》
CSCD
北大核心
2009年第5期415-420,共6页
Acta Botanica Yunnanica
基金
贵州省凯里学院植物学重点学科基金资助(2008ZW002)
