反相高效液相色谱法测定鱼肉组织中四环素族抗生素残留量

Determination of tetracyclines residues in fish meat by RP-HPLC

建立了一种采用固相萃取-反相高效液相色谱法同时测定鱼肉中土霉素、四环素、金霉素的分析方法,即样品用McI-Vaine-EDTA溶液提取,C18小柱净化,流动相洗脱定容,选择355nm为检测波长进行检测,结果表明:土霉素、四环素、金霉素的检出限分别为0.02、0.03、0.04mg/kg,方法相对标准偏差为2.95%～5.73%,平均回收率为80%～92%。

Abstract： A sensitive method for simultaneously determining residues of tetracyclines oxytetracycline, tetracycline and chlortetracycline in fish meat was established by RP- HPLCwith solid phase extraction. Samples were extracted with Na2EDTA- Mcllvaine buffer solutions and centrifuged. The sample extracts were cleaned up by C18 solid-phase extraction cartridge and then were determined by detection at 350 nm.Under the selected conditions the detection limits were 0.02 mg/kg, 0.03 mg/kg and 0.04 mg/kg for oxytetracycline,tetracycline and chlortetracycline, respectively. The RSDs of 2.95%-5.73% ,and average recoveries of 80%-92% were obtained for them.