c-Abl调控FHL2的转录活性

c-Abl Regulate the Transcriptional Activity of FHL2

目的:研究c-Abl对FHL2转录活性的调控。方法:应用免疫共沉淀和免疫印迹验证c-Abl与FHL2的相互作用及c-Abl对FHL2的磷酸化作用,并用萤光素酶报告基因研究c-Abl对FHL2转录活性的调控。结果:c-Abl与FHL2结合并磷酸化FHL2,促进FHL2的转录活性,并与FHL2一起促进FHL2下游因子骨钙蛋白的基因启动子活性。结论:c-Abl能促进FHL2的转录活性。

Objective：To investigate the effect of c-Abl on FHL2 transcription activity.Methods：The interaction of c-Abl and FHL2 was demonstrated by co-immunoprecipitation and Western blotting,the FHL2 transcription activity was detected by luciferase reporter system.Results：c-Abl bind with and phosphorylate FHL2,and increase the promoter activity of osteocalcin,one target gene of FHL2.Conclusion：c-Abl could promote the FHL2 transcription activity.