水稻Ds插入淡绿叶突变体的鉴定和遗传分析

Identification and genetic analysis of pale-green mutant caused by Ds insertion in rice

Ac/Ds插入突变是水稻基因功能鉴定的有力工具之一。文章从水稻中花11Ds-T-DNA转化纯合体与Ac-T-DNA转化纯合体的杂交群体中筛选到一个淡绿叶突变体。该突变体在三叶期由绿苗转为淡绿叶苗,自然光照下突变体迅速焦枯,但是在弱光照条件下,突变体能缓慢生长至开花结实;突变体光合作用特性研究表明该突变是典型的光抑制突变体。遗传分析表明该突变为Ds插入导致的隐性突变。

Ac/Ds insertion mutation was thought to be one of the powerful tools for identifying gene function in rice. In this study, the rice mutant with pale-green leaves was isolated from the population of Ds-T-DNA and Ac-T-DNA transgenic homozygote in a japonica rice variety Zhonghua 11. The leaves of the mutant turned to be pale-green at three-leaf stage. This mutant was capable of growing slowly and maturing under low-light conditions, but, rapidly died under natural light conditions. The analysis of the photosynthetic activity characteristics by measuring chlorophyll fluorescence in vivo suggested that the mutant was a typical photo-inhibitory mutant. Genetic analysis demonstrated that the mutation was the recessive one resulted from Ds insertion.