家兔实验性血栓模型的建立及其检测

The preparation of rabbit's artery thrombus model and its detection

目的:建立一种简单、快速而又非闭塞性的动脉血栓模型。方法:采取化学损伤-三氯化铁(FeCl3)诱导的方法来制备兔颈总动脉血栓模型。取9只家兔根据FeCl3浸润时间随机分成3组:20min(第1)组、40min(第2)组、60min(第3)组每组各3只。结果:FeCl3腐蚀法形成的血栓为一种混合型血栓;第2组模型血管管腔可见非闭塞性血栓形成,血栓占管腔1/4～1/3;第3组模型可见闭塞性血栓形成,血栓占据整个管腔。结论:FeCl3诱发的血栓模型可靠、形成简便、快速,结构更接近于临床自发性血栓形成的组织学形态特征,为血栓性疾病的研究提供了很好的方法。

Objective：To set up a kind of arterial thrombus model which is simple, rapid and non-obstructive. Method：Rabbit thrombus model made by FeCl3 infiltration in common carotid artery of rabbit was set up. Nine rabbits were randomly invided into three groups according to FeCl3 infiltration time： 20min （the first group）, 40 min （the second group）, 60 min （the third group）. Result： rabbit thrombus model made by FeCl3 infiltration in common carotid artery of rabbit was set up, the second group： non-obstructive thrombosis and thrombus holds about 1/3-1/4 of vessel： the third group： obstructive-occluding thrombosis and thrombus holds the total vessel, and the thrombosis is mixed thrombus. Conclusion：The type of thrombus resulted from FeCl3 erosion is mixed pattern,which is similar to spontaneous thrombus in clinic.