内皮素-1反义脱氧寡核苷酸对糖尿病肾病大鼠肾组织形态学的影响

Protective effects of endothelin-1 mRNA antisense oligodeoxynucleotide on the kidney ultrustructure of streptozotocin-induced diabetic nephropathy rats

目的:研究内皮素-1反义脱氧寡核苷酸(ET-1AS-ODN)对链脲佐菌素(streptozotocin,STZ)诱导的糖尿病大鼠肾组织形态学的保护作用及其机制.方法:一次性腹腔注射STZ(60mg/kg),建立大鼠糖尿病模型,观察ET-1AS-ODN预防性给药对大鼠肾组织形态结构的保护作用.结果:ET-1AS-ODN可明显减轻糖尿病大鼠肾肥大指数,改善肾组织形态学损伤.结论:内皮素-1反义寡核苷酸可减轻STZ诱导的糖尿病肾病大鼠的肾损伤,对肾组织具有保护作用.其机制与减少内源性内皮素的产生有关.

AIM： To investigate the protective effects of endothelin-1 mRNA antisense oligodeoxynucleotide （ET-1 AS-ODN ） on the renal morphology of diabetic nephropathy（DN） and discuss the pathogenesis and mechanism of DN in a diabetic rat model. METHODS： Diabetic rats were induced by intraperitoneal administration of streptozotocin （STZ） at 60 mg/kg. ET-1 AS-ODN was administrated intravenously to the treatment groups and its protective effects on renal morphology were observed. RESULTS： Compared with the DM group, the kidney index reduced significantly the general condition and morphological injury improved significantly in ET-1 AS-ODN treatment groups. CONCLUSION： Endothelin-1 mRNA antisense oligodeoxynucleotide has a protective effect on the kidney by preventing renal impairment in diabetic nephropathy rats. The mechanism of the protective effect of Endothelin-1 mRNA antisense oligodeoxynucleotide involves inhibition of endogenous ET-1 production.