细粒棘球绦虫(中国大陆株)诊断抗原P-29重组蛋白的免疫保护性研究

Protective immunity of recombinant EgP-29 against Echinococcus Granulosus in mice

目的:探讨细粒棘球蚴(Eg)诊断抗原P-29重组蛋白的免疫保护性及其作为候选疫苗的潜在价值.方法:ICR小鼠随机分为蛋白免疫组和佐剂对照组,每隔2wk皮下免疫1次,在第3次免疫后2wk,用Eg原头蚴进行攻击感染,感染后20wk剖杀小鼠,检获棘球蚴包囊,计算免疫保护力,并用ELISA法测定血清中IgG及其亚型和IgE水平.结果:与佐剂对照组比较,蛋白免疫组小鼠的免疫保护力为96.6%;与免疫前比较,免疫后和攻击感染后蛋白免疫组小鼠血清IgG,IgG1,IgG2a和IgE水平均明显升高(P<0.05),IgG2b降低(P<0.05).结论:细粒棘球绦虫诊断抗原P-29重组蛋白能诱导小鼠产生一定的保护性免疫,是潜在的疫苗候选抗原分子.

AIM： To investigate the protective immunity against Echinococcus granulosus in mice immunized with rEgP-29 and its potential as vaccine candidate. METHODS： ICR mice were subcutaneously immunized three times with rEgP-29 and then challenged with Echinococcus granulosus, The rate of reduced hydatid cyst and the levels of IgE, IgG and IgG subclasses in sera were examined, with adjuvant group as control. RESULTS： The rate of reduced hydatid cyst in rEgP-29 group was 96.6%. The levels of IgG, IgG1, IgG2a and IgE increased obviously, that of IgG2b decreased remarkably. CONCLUSION： The rEgP-29 antigen could induce partial protection against Echinococcus granulosus. These data demonstrate the potential of rEgP-29 as a Echinococcus granulosus vaccine candidate.