国产无机三氧化物聚合物的生物相容性研究

Studies on biocompatibility of domestic MTA

目的:评价新材料国产无机三氧化物聚合物(MTA)的生物相容性,以判断所制材料是否满足相应的生物安全性标准,并与进口MTA进行对比.方法:应用扫描电镜(SEM)观察新材料国产MTA的表面形貌;通过MTT法检测细胞相对增殖率,对材料的细胞毒性进行分级评价,并与进口MTA进行比较;利用SEM观察体外培养的细胞在材料表面黏附与增殖情况.结果:MTT结果显示国产MTA与进口MTA两组数据差异显著(P<0.05),但细胞毒性均为一级;成骨细胞在材料表面具有较好的细胞伸展形态,可以成功的贴附生长在材料上并进行增殖.结论:国产MTA具有良好的生物相容性.

AIM： To evaluate the biocompatibility of domestic mineral trioxide aggregate （MTA） and determine whether this new material complies with the related Bio-safety Standards. METHODS ： The surface microtopograph of the new material（ domestic MTA） was observed through scanning electron microscope （SEM）; Cell relative growth rate （RGR） measured by MTT methods was used as quantitative assessment for cytotoxicity of the biomaterial, and compared with imported MTA; SEM was used to characterize surface microtopograph of osteoblast ceils attach and proliferate on the material. RESULTS： The result of MTT indicated that there was obviously significant difference between the domestic group and imported MTA group （ P 〈 0.05 ）, their cytotoxicity were both at grade 1. Osteoblast cells spread well on the material, could successfully be adhered to the material and proliferate. CONCLUSION： The new material ＇domestic MTA） shows good biocompatibility.