棉铃虫磷酸甘油醛异构酶基因Hatpi的克隆及分析(英文)

Isolation and analysis of the triosephosphate isomerase gene,Hatpi,from Helicoverpa armigera

棉铃虫Helicoverpa armigera是一种严重危害棉花等经济作物的鳞翅目害虫,开展分子水平研究对防控害虫将具有重要参考意义。本研究利用RACE(rapid amplification of cDNA ends)技术克隆了棉铃虫磷酸甘油醛异构酶(triosephosphate isomerase)基因Hatpi(GenBank登录号为AY736358)。该基因cDNA全长为1149bp,编码248个氨基酸,预测等电点为5.82,分子量为16.4kD。HaTPI含有磷酸甘油醛异构酶类蛋白的典型(βα)8结构、保守的活性位点(Lys12,His94和Glu165)和小肽序列(AYEPVWAIGTG和GGASLKPEF)等。RT-PCR检测分析发现Hatpi在棉铃虫卵巢、幼虫、蛹、成虫均有表达,提示该基因可能在棉铃虫的不同发育阶段均起作用。

Helicoverpa armigera is an important lepidopteran pest of many crops including cotton. Molecular analysis will promote pest control in the future. The gene that encoding triosephosphate isomerase in Helicoverpa armigera, Hatpi, was molecularly cloned and analyzed in this study. The cDNA of Hatpi （ GcnBank accession no. AY736358 ） is 1 149 bp in length and encodes a 248 amino acid protein with the estimated molecular mass of 26.4 kD and isoelectric point of 5.82. （i3ct） 8structure found in the deduced protein structure of HaTPI was similar to that of other triosephosphate isomerase. High identity was also found in the active catalytic sites （Lys12, His94, and Glu165） and peptide motifs （AYEPVWAIGTG and GGASLKPEF）. RT-PCR analysis results showed that Hatpi was expressed in the embryo, larva, pupa and adult of H. armigera, suggesting Hatpi may play roles in various developmental stages of H. armigera.