急性肝衰竭大鼠血及胃肠黏膜组织中NT,CGRP含量的变化

Diversities in expression of neurotensin and calcitonin gene-related peptide in hematological system and gastrointestinal mucosa of rats with acute liver failure

目的:探讨神经降压素(NT)、降钙素基因相关肽(CGRP)在急性肝衰竭(ALF)大鼠时的含量变化及其与肝性胃肠功能障碍的相关性研究.方法:用硫代乙酰胺(TAA)复制出ALF大鼠模型.观察其一般情况、肝脏生化指标、放射免疫分析法(RIA)测定其血浆及胃肠黏膜组织中NT和CGRP含量并与正常对照组相比.结果:①模型组大鼠血浆中及空肠黏膜组织中NT,CGRP含量及胃窦黏膜中NT含量明显升高,与正常对照组比较差异有统计学意义,(P<0.01,P<0.05),胃窦黏膜中CGRP含量无明显变化;②直线相关性分析显示:模型组大鼠血浆NT,CGRP含量与丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)水平之间呈显著正相关(r分别为0.56,0.62,0.68,0.70,均P<0.05);③模型组大鼠分别于造模后(12,24,36,48h)随ALF病情的进展NT,CGRP含量呈进行性升高,其含量于造模后48,12h相比有显著性升高(P<0.01).结论:测定血浆NT,CGRP含量可作为判断肝功能损伤的指标之一;对于判断ALF的病情、肝性脑病、肝性胃肠功能障碍及预后有一定的指导意义.

AIM：To discuss the diversities in expression of neurotensin（NT）and calcitonin gene-related peptide（CGRP）in rats with acute liver failure（ALF）and its associativity with hepatic gastrointestinal dysfunction.METHODS：We used thioacetamide（TAA）to induce ALF in rat models and observed their general state of health and liver biochemical index.The expressions of NT and CGRP in the hematological system and gastrointestinal mucosa of ALF rat models were identified by the results of radioimmunoassay and compared with the normal control group.RESULTS：①The expressions of NT and CGRP in the hematological system and intestinal mucosa and the expressions of NT in the gastric mucosa of the ALF rats were significantly elevated compared with the normal control group（P〈0.01,P〈0.05）,whereas the expression of CGRP in gastric mucosa showed no significant change（P〉0.05）.②The linear regression analysis demonstrated a positive correlation between hematic levels of NT and CGRP and levels of ALT and ASTr was 0.56,0.62,0.68,0.70,respectively,P〈0.05）.③With serial NT and CGRP samples at time line 12,24,36 and 48 h,the expressions of NT and CGRP increased progressively releventing the degree of the disease,and were significantly higher at 48 h than at 12 h（P〈0.01）.CONCLUSION：The expressions of NT and CGRP in hematological system can be used as a marker for liver function and have some significant judgement of the condition of ALF and the prognosis of hepatic encephalopathy and gastrointestinal dysfunction.It is worth to analysis further if that can be a useful model for early diagnosis of hepatic encephalopathy and gastrointestinal dysfunction.