abeM基因与鲍曼不动杆菌多重耐药性的关系

Relationship of multidrug-resistance with AbeM gene in clinical isolates of Acinetobacter baumannii

目的:探讨abeM基因与临床分离的鲍曼不动杆菌多重耐药性的关系.方法:采用琼脂稀释法检测8种抗菌药物对37株鲍曼不动杆菌的最低抑菌浓度(MICs);以PCR和RT-PCR检测abeM基因的分布及其表达水平.结果:临床分离的鲍曼不动杆菌耐药性高,37株临床分离的鲍曼不动杆菌中,耐药菌株有21株(56.76%),且多重耐药菌株占耐药菌株总数的81%.所有临床分离株均能检测到abeM基因,但多重耐药菌株abeM基因mRNA表达水平显著高于敏感菌株(P<0.01),也明显高于仅对2类或1类抗菌药物耐药的菌株(P<0.05).结论:abeM基因的高表达与临床分离的鲍曼不动杆菌多重耐药性密切相关.

AIM：To explore the relationship of multidrug-resistance with abeM gene in the clinical isolates of Acinetobacter baumannii.METHODS：Agar dilution method was used to determine the minimum inhibitory concentrations（MICs）of 8 antimicrobials against 37 isolates of Acinetobacter baumannii.AbeM gene and its mRNA expression in Acinetobacter baumannii isolates were detected by PCR and RT-PCR,respectively.RESULTS：The clinical isolates of Acinetobacter baumannii were generally resistant to antimicrobials.Among 37 isolates of Acinetobacter baumannii,21 strains are resistant（56.76%）and the multidrug-resistant strains account for 81% in resistant ones.AbeM gene was found in all isolates and the levels of mRNA expression of abeM gene in multidrug-resistant strains were significantly higher compared with the susceptible ones（P〈0.01）and general resistant ones（P〈0.05）.CONCLUSION：High level expression of abeM gene was immediately associated with the multidrug-resistance in the clinical isolates of Acinetobacter baumannii.