摘要
目的:观察脉冲电磁场对去势大鼠骨髓源性破骨细胞样细胞(OLC)形成变化及凋亡的影响。方法:30只健康3月龄雌性Wistar大鼠,随机分为A组(双侧卵巢切除+磁场治疗组、18只)、B组(双侧卵巢切除组、6只)和C组(伪去势组、6只)。A组又随机均分3组:A1、A2、A3组,电磁场频率分别为1.5、2、75Hz。术后单纯喂养3个月后对各组大鼠骨髓细胞进行培养,培养2d后,A1、A2、A3组置脉冲电磁场治疗,1次/d,每次30min,共治疗2周。之后各组培养细胞作瑞氏-吉姆萨、抗酒石酸酸性磷酸酶(TRAP)和Hoechst 33258染色,观察破骨细胞变化并计数。结果:大鼠骨髓体外培养细胞TRAP染色结果显示,C组的破骨细胞形成数量最少,其次为A2、A3、A1、B组。B组的破骨细胞形成数量与C组和A2组比较显著增多(P<0.01),B组的破骨细胞形成数量与A1、A3组比较明显增多(P<0.05)。Hoechst 33258染色结果显示,C组破骨样细胞凋亡出现数量较多,C、A2组凋亡数明显多于B组(P<0.05)。结论:脉冲电磁场能使大鼠骨髓体外培养破骨细胞形成数量减少、凋亡数增加,频率为2Hz的效果最佳,表明脉冲电磁场可能将是骨质疏松症治疗的新方法。
Objective:To investigate the effect of pulsed electromagnetic fields (PEMs) on inducing osteoclastic like cell (OLC) formation changes and apoptosis in ovariectomized (OVX) rats bone marrow culture in vitro. Methods:Thirty healthy three month old female Wistar rats were either sham operated(Sham) or ovariectomized(OVX)and randomly divided into three groups:group A (OVX+PEMs,18 rats),group B (OVX,6 rats) and group C (Sham,6 rats);group A was again randomly divided into three groups:A1、A2、A3. The frequencies adopted were 1.5,2,75 Hz and 30 minutes for once a day. All rats were fed with normal diet for 3 months,then the bone marrow of all rats were cultured,2 days later,group A cells (including group A1,A2,A3) were collected and exposed to different frequencies PEMs for 2 weeks (30 min / day). In order to observe the changes of osteoclasts and count their numbers,cells were taken for Wright Giemsa staining,tartrate resistance acid phosphatase(TRAP) staining and Hoechst 33258 staining. Results:TRAP staining results indicated the number of OLC in group C was the least,then was group A2,A3,A1,B. The number of OLC in group B was remarkly increased(P0.01;vs group C,A2). The number of OLC in group B was significantly increased(P0.05;vs group A1,A3). Hoechst 33258 staining results indicated the number of apoptosis of OLC in group C was more than other groups,which of group C,A2 was significantly increased (P 0.05;vs group B). Conclusion:PEMs had decreased the formation of OLC and increased the number of apoptosis of OLC in ovariectomized (OVX) rats bone marrow culture in vitro,the effects of 2 Hz was the best. PEMs would be a new way of osteoporosis therapy.
出处
《中国骨伤》
CAS
2009年第10期727-729,共3页
China Journal of Orthopaedics and Traumatology
关键词
卵巢切除
大鼠
脉冲电磁场
破骨细胞
细胞凋亡
Ovariectomy
Rats
Pulsed electromagnetic fields
Osteoclasts
Apoptosis