水中草颗粒对实验动物复发性口腔溃疡细胞免疫功能的影响

Effect of Shuizhongcao Granule on Cellular Immune Function of Experimental Animal with Recurrent Aphthous Stomatitis

目的探讨水中草颗粒对复发性口腔溃疡细胞免疫功能的影响。方法采用免疫方法建立复发性口腔溃疡动物模型,将成模的复发性口腔溃疡家兔随机分为模型组、左旋咪唑组(2.5mg/mL)、水中草低剂量组(0.25g/mL)和高剂量组(0.5g/mL),同时设立正常组。从实验的第7周开始,左旋咪唑组、水中草低剂量组及水中草高剂量组每天分别以相应药物灌胃,共连续28天;取耳缘静脉血检测T淋巴细胞亚群(CD3、CD4、CD8)、血清白细胞介素(IL)-10和IL-12的水平。结果模型组家兔CD3、CD4及CD4/CD8比值[分别为(40.50±7.46)%、(30.80±5.33)%及79.56±8.32]均较正常组显著下降(P<0.05),血清IL-10水平[(8.02±0.53)ng/L]较正常组升高(P<0.05),IL-12水平[(15.51±1.35)ng/L]较正常组下降(P<0.05);血清CD3、CD4、CD4/CD8比值及IL-12水平,左旋咪唑[(55.30±6.33)%、(43.50±5.33)%、99.29±13.84及(17.72±1.70)ng/L]、水中草低剂量[(58.50±6.03)%、(42.80±3.17)%、110.27±12.85及(17.74±1.96)ng/L]、水中草高剂量组[(61.50±4.32)%、(47.20±4.37)%、103.30±7.42及(18.18±1.54)ng/L]均较模型组升高(P<0.05,P<0.01),血清IL-10水平左旋咪唑组、水中草低剂量组和水中草高剂量组均较模型组降低(P<0.05,P<0.01)。结论复发性口腔溃疡家兔免疫功能处于抑制状态,水中草颗粒能够改善复发性口腔溃疡家兔的免疫抑制状态,达到治疗复发性口腔溃疡的作用。

Objective To investigate the effect of Shuizhongcao Granule（SZCG）on cellular immune function in animal model of recurrent aphthous stomatitis（RAS）.Methods Experimental animal model of RAS were established by immunological method.The modeled animals were randomized into 4 groups,Group A was the model control group,Group B was treated by Levamisole（2.5 mg/mL）,Group C and D was treated with high-dose（0.5 g/mL）and low-dose（0.24 g/mL）SZCG respectively.The medication was administered via gastric perfusion,starting from the 7th week of the experiment,and continued for 28 days.Meanwhile,a normal control group was set up.Percentage of T-lymphocyte subsets,CD3,CD4 and CD8,and serum levels of interleukin 10 and 12（IL-10 and IL-12）in peripheral blood were detected before and after treatment.Results Percentages of CD3,CD4 and CD4/CD8 ratio in the model control group were 40.50±7.46%,30.80±5.33% and 79.56±8.32 respectively,all lower than those in the normal control group（P〈0.05）;level of serum IL-10 was higher（8.02±0.53 ng/L）and that of IL-12 was lower（15.51±1.35 ng/L）in the model animal than those in normal control（P〈0.05）.After treatment,levels of CD3,CD4,CD4/CD8 and IL-12 in Group B were 55.30±6.33%,43.50±5.33%,99.29±13.84 and 17.72±1.70 ng/L,those in Group C were 61.50±4.32%,47.20±4.37%,103.30±7.42 and 18.18±1.54 ng/L,and in Group D were 58.50±6.03%,42.80±3.17%,110.27±12.85 and 17.74±1.96 ng/L,respectively,all were higher than those in Group A,but levels of IL-10 in the three treatment groups were lower than that in Goup A（P〈0.05 or P〈0.01）.Conclusion The immune function of RAS model animals is in the suppressed condition;SZCG can improve the immune suppression to achieve its therapeutic effect on the disease.