聚电解质复合法载表阿霉素纳米微粒的制备及抗胰腺癌作用的研究

目的合成载表阿霉素纳米微粒(E-ADM-NPs)并检测其相关参数,观察其对人胰腺癌细胞的杀伤作用。方法采用聚电解质复合法合成E-ADM-NPs,动态光散射粒子分析仪测定粒径,分光光度法计算包封率,噻唑蓝(MTT)法观察对胰腺癌细胞的体外杀伤作用。结果E-ADM-NPs的平均粒径(185±23)nm,包封率(76.2±6.5)%,体内抑瘤实验表明E-ADM-NPs的疗效优于未包载表阿霉素(unenveloped epirubicin,E-ADM)。

Objective To prepare E-ADM-NPs. detect their correlative parameters and explore their anti-pancreatic carcinoma effect. Methods The E-ADM-NPs were prepared by the method of polyelectrolyte complexation. The diameter of the nanoparticles was determined by dynamic light scattering particle analysator. The embedding ratio of E-ADM-NPs was calculated by spectrophotometer. The inhibitory effect of the particles on pancreatic carcinoma cells was explored by MTT assay in vitro. Results The mean diameter of E-ADM-NPs was （185-23）nm, the embedding ratio was （76.2±6.5）%. The in vivo tests manifested that the efficacy of E-ADM-NPs had advantages over E-ADM. Conclusion As a novel and safe kind of drug sustained release system, E-ADM-NPs can effectively inhibit the growth of pancreatic carcinoma cells.